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Innovation

External Collaboration

Advances are made daily in safety research. To be able to conduct evaluations in the most appropriate way, it is essential to acquire the most-up-to-date technologies and information and to share Shiseido's technologies with experts. That is, researchers in safety assurance at Shiseido have been working with outside experts in a wide range of activities. Some of those activities are described below.

Various collaborative activities with individuals outside the company

・Interactions with outside experts

Shiseido regularly undertakes consultations on safety with Japanese and foreign experts who are active in safety assurance. Moreover, as a cosmetic company, Shiseido takes the opinions of dermatologists seriously. We actively participate in safety-related activities held by academic societies.

・Industry activities

Shiseido takes part in events held by cosmetic industry associations around the world (e.g., Japan Cosmetic Industry Association, Cosmetics Europe (CE)(opens in new window.) , Personal Care Products Council (PCPC)(opens in new window.) , ASEAN Cosmetic Association)(opens in new window.) , and actively exchanges information related to safety. Shiseido also actively contributes to safety-related activities organized by relevant industry associations (e.g., Japan Chemical Industry Association, Japan Soap and Detergent Association, Japan Hair Color Industry Association).

・Development of professional human resources

Researchers working in safety assurance regularly receive training at universities and public research institutions and take part in symposiums and education seminars held outside of the company to improve their levels of professional expertise and expert knowledge.

・Basic research on safety

Shiseido has also been collaborating with outside organizations on research topics that are the foundations of safety, such as sensory irritation, sensitive skin, and the development of hypo-irritant compounds, and the results have been useful in our efforts to develop safe, secure and reliable products.

Shiseido’s commitment on alternatives to animal experiments

・Sharing of research findings

Shiseido shares with the public the findings obtained from years of research by publishing them on this website, in the literature and in academic conferences. In addition, Shiseido also makes all test methods available for external use, including the patented core technology of h-CLAT, developed jointly with Kao Corporation (all royalties from the core technology of h-CLAT have been donated to Japanese Society for Alternatives to Animal Experiments). Through such efforts, alternative test methods developed with Shiseido are expected to be widely used in a greater number of research institutions and are expected to see further improvements. For any inquiries regarding this issue, please click here
Reference:Initiatives in Response to Animal Testing and Alternative Methods

・Further research on alternative methods

Efforts to find alternative methods are ongoing at Shiseido with the aim of further improving precision and expanding the domain of applicability. Shiseido has also taken on the challenge in developing alternative in vitro methods for evaluating long-term systemic toxicity.

・Efforts on establishing officially acceptable methods and industry standards

Shiseido has been pushing for the establishment of officially acceptable alternative methods for eye irritation and skin sensitization testing. Specifically, Shiseido has been taking part in the validation activities of the Japanese Center for the Validation of Alternative Methods (JaCVAM)(opens in new window.) and the European Union Reference Laboratory for Alternatives to Animal Testing (EURL ECVAM)(opens in new window.) and working as a member of the OECD’s expert team to draw up guidance proposals. The h-CLAT was listed on Economic Co-operation and Development (OECD) test guideline in July 2016. In addition, Shiseido has also been actively participating in the industry’s efforts to standardize alternative methods and to increase public awareness.

Year Shiseido’s studies that have contributed to the development of alternative methods and safety evaluation
2016 ・Safety assessment of Cosmetic Ingredients by Hand1-Luciferase Embryonic Stem cell Tests
・Application of photo-KeratinoSens to Integrated Testing Strategy of photosensitization
・In silico risk assessment for human percutaneous absorption and skin sensitization of drug
・In silico risk assessment for skin sensitization using artificial neural network analysis
2015 ・Specificity evaluation of Embryonic Stem cell Test for cosmetic ingredients
2014
Development of h-CLAT, an alternative to skin sensitization test (jointly awarded the JCIA Special Award in Technology with Kao Corporation)
・Development of h-CLAT, an alternative to skin sensitization test
(jointly awarded the JCIA Special Award in Technology with Kao Corporation)
2011 ・Study on cell-surface SH groups as a marker in an alternative skin sensitization test (SH test)
2008 ・Study on an eye irritation evaluation method based on a combination of SIRC-CVS test and the three-dimensional cultured skin model test
2005 ・Joint study in Japan on the in vitro skin sensitization test: h-CLAT (human Cell Line Activation Test)
・Study on the effect of light in in vitro phototoxicity tests
2003 ・Study on alternative methods of acute toxicity testing using cultured cells
2002 ・Study on the development of a formula for predicting percutaneous absorption and its application in predicting sensitization potentials
2001 ・Study on an in vitro sensitization test that measures the expression of CD86 and MHC class II as markers in the THP-1 human monocytic cell line
2000 ・Study on the induction of unscheduled DNA synthesis in the epidermis by skin carcinogens
1999 ・Study on cell line differences in photocytotoxicity tests
1997 ・Study on the use of the cytotoxicity test of poorly soluble substances as an alternative method for skin irritation testing
1996 ・Study on the use of quantitative measurement of pro-inflammatory cytokine gene expression as an alternative method for skin irritation testing
1994 ・Study on the application of electrophysiological techniques and other techniques on neuronal cells for use as an in vitro sensory irritation test
1992 ・Study on in vitro phototoxicity tests with red blood cells and yeasts
1991 ・Study on an in vitro test of eye irritation using hemoglobin as a biomarker
・Study on the use of liposomes prepared from lipids extracts of the cornea as an alternative for eye irritation testing

<Studies and other works that have received awards from the Japanese Society
for Alternatives to Animal Experiments, among other organizations>

Academic conference presentations(2013~2018)

  • The 31st Annual Meeting of the Japanese Society for Alternatives to Animal Experiments, 2018
    • Analysis of the threshold of toxicological concern in each organ for assessing the systemic toxicity
    • Photosensitization evaluation system using WoE ~ Construction of human database ~
    • Weight of Evidence approach to assess photosensitization potential (Improvement of photo-h-CLAT method)
  • The 45th Annual Meeting of the Japanese Society of Toxicology, 2018
    • Development of in silico systemic toxicity assessment method for cosmetic raw materials
    • Mechanism based integrated assay systems for the prediction of drug induced liver injury
  • The 43th Annual Meeting of the Japanese Cosmetic Science Society, 2018
    • Threshold of toxicological concern in cosmetic use
  • Society of Toxicology 57th Annual Meeting, 2018
    • An approach for evaluating the skin sensitization of cosmetic ingredients using non-animal safety assessment methods
  • The 2018 International Joint Meeting of the 22nd International Symposium on MDO(Microsomes and Drug Oxidations) and the 33rd Annual Meeting of JSSX(The Japanese Society for the Study of Xenobiotics), 2018
    • Needs meet Seeds -Unlimited potential of liver microphysiological system-
  • 10th World Congress on Alternatives and Animal Use in the Life Sciences, 2017
    • Development of in vitro hepatotoxicity assay system focusing the drug induced liver injury(DILI) mechanism
  • Society of Toxicology 56th Annual Meeting, 2017
    • Development of risk assessment models for skin sensitization based on an artificial neural network analysis of human Cell Line Activation Test (h-CLAT), Direct Peptide Reactivity Assay (DPRA), KeratinoSensTM and in silico parameter
  • IFSCC, 2017
    • Prediction of concentrations of cosmetic ingredients in the blood and organs after topical application to the skin
  • Society of Toxicology 55th Annual Meeting, 2016
    • Development of photo Direct Peptide Reactivity Assay
  • Society of Toxicology 54th Annual Meeting, 2015
    • Verification of a skin sensitization assessment neural network model by fragrance materials
  • 9th World Congress on Alternatives and Animal Use in the Life Sciences, 2014
    • Photo-safety assessment using multiple in vitro tests
  • IFSCC, 2014
    • Application of Reactive Oxygen Species (ROS) assay for photosafety evaluation of cosmetic ingredients
  • Society of Toxicology(opens in new window.) 52nd Annual Meeting, 2013
    • Safety assessment of cosmetics using alternative methods
      -skin sensitization evaluation-
    • Approach to photoallergenicity assay for cosmetic ingredients
      using non-animal methods
  • International Congress of Toxicology, 2013
    • Development of a non-animal safety assessment system in Shiseido



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